- The cost of making 100 amplicon libraries and pooling them for a single MiSeq PE250 run is approximately $1700. This includes the cost for DNA extraction, PCR primers and reagents, and the sequencing.
- The cost of making 24 (meta)genomic libraries, using the Nextera XT kit (required for low-input samples) and pooling them for a single MiSeq PE250 run is approximately $2100.
- The cost of making 24 (meta)genomic libraries, using the TruSeq kit (preferred for high-input samples) and pooling them for a single MiSeq PE250 run is approximately $2600.
The above estimates are based on the numbers provided below, plus a little bit of rounding up for incidentals and labor and such.
HiSeq 2500 – SR50 (Single Read 50 cycles) $905
HiSeq 2500 – PE100 (Paired End 100 cycles) $2040
MiSeq PE150 (300 cycles + indexes) $1100
MiSeq PE250 (500 cycles + indexes) $1179
Nextera XT-DNA Sample Prep Kit (96) samples $2,465.00
Nextera XT Index Kit -96 barcode sets $807.50
Nextera XT-DNA Sample Prep Kit (24) samples $658.75
Nextera XT Index Kit -24 indices, 96 samples $212.50
TruSeq Kit v2, set A (48 samples) $2,210.00
PCR reagents (250 reactions) ~$250
Barcoded PCR primers w/ Illumina adaptors ~$7 each
Agenocourt AMPure XP Beads 60 ml (A63881), $1104
PCR tubes (1000) $78
MoBio Power Soil DNA extraction kit (96 extractions) $250