Sequencing Costs

1.  The cost of making 100 amplicon libraries and pooling them for a single MiSeq PE250 run is approximately $1700. This includes the cost for DNA extraction, PCR primers and reagents, and the sequencing.
2. The cost of making 24 (meta)genomic libraries, using the Nextera XT kit (required for low-input samples) and pooling them for a single MiSeq PE250 run is approximately $2100.
3. The cost of making 24 (meta)genomic libraries, using the TruSeq kit (preferred for high-input samples) and pooling them for a single MiSeq PE250 run is approximately $2600.

The above estimates are based on the numbers provided below, plus a little bit of rounding up for incidentals and labor and such.

HiSeq 2500 – SR50 (Single Read 50 cycles)        $905

HiSeq 2500 – PE100 (Paired End 100 cycles)        $2040

MiSeq PE150 (300 cycles + indexes)        $1100

MiSeq PE250 (500 cycles + indexes)        $1179

Nextera XT-DNA Sample Prep Kit (96) samples         $2,465.00

Nextera XT Index Kit -96 barcode sets                            $807.50

Nextera XT-DNA Sample Prep Kit (24) samples         $658.75

Nextera XT Index Kit -24 indices, 96 samples                   $212.50

TruSeq Kit v2, set A (48 samples)                            $2,210.00

PCR reagents (250 reactions)       ~$250

Barcoded PCR primers w/ Illumina adaptors      ~$7 each

Agenocourt AMPure XP Beads 60 ml (A63881),  $1104

PCR tubes (1000)       $78

MoBio Power Soil DNA extraction kit (96 extractions)    $250