- The cost of making 100 amplicon libraries and pooling them for a single MiSeq PE250 run is approximately $1700. This includes the cost for DNA extraction, PCR primers and reagents, and the sequencing.
- The cost of making 24 (meta)genomic libraries, using the Nextera XT kit (required for low-input samples) and pooling them for a single MiSeq PE250 run is approximately $2100.
- The cost of making 24 (meta)genomic libraries, using the TruSeq kit (preferred for high-input samples) and pooling them for a single MiSeq PE250 run is approximately $2600.
The above estimates are based on the numbers provided below, plus a little bit of rounding up for incidentals and labor and such.
HiSeq 2500 – SR50 (Single Read 50 cycles) $905
HiSeq 2500 – PE100 (Paired End 100 cycles) $2040
MiSeq PE150 (300 cycles + indexes) $1100
MiSeq PE250 (500 cycles + indexes) $1179
Nextera XT-DNA Sample Prep Kit (96) samples $2,465.00
Nextera XT Index Kit -96 barcode sets $807.50
Nextera XT-DNA Sample Prep Kit (24) samples $658.75
Nextera XT Index Kit -24 indices, 96 samples $212.50
TruSeq Kit v2, set A (48 samples) $2,210.00
PCR reagents (250 reactions) ~$250
Barcoded PCR primers w/ Illumina adaptors ~$7 each
Agenocourt AMPure XP Beads 60 ml (A63881), $1104
PCR tubes (1000) $78
MoBio Power Soil DNA extraction kit (96 extractions) $250
2 thoughts on “Sequencing Costs”
We would like to sequence the whole metagenome of different microbial consortia, can you provide the service to external institutions?
We can help people do this type of work but don’t do such things as a service per se (interested in collaborations where we are involved in the science). Send email to jonathan dot eisen at gmail dot com