Introduction to Flow Cytometry for Aquatic Sciences
September 12-16, 2016
Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA
Course information and Registration:
This course is organized by Dr. Nicole Poulton, Director of the J.J. MacIsaac Facility for Aquatic Cytometry (https://fac.bigelow.org) and will include additional lecturers who are experts within their fields. Registration is limited to 10 participants.
The 5-day course will include both lecture and laboratory sessions, and provides aquatic and environmental scientists and/or commercial entities an introduction to flow cytometry, including the theory of operation as well as an introduction to different flow cytometric instrumentation (analyzers and cells sorters) including imaging cytometry tools (FlowCAM). Both breakfast, coffee breaks and lunch are provided on all days and will also include one group dinner with the lecturers (Maine Lobster Bake). Lodging is not included.
Throughout the week topics will include discussions on the wide number of applications within the aquatic sciences including, but not limited to:
1. Culture & Environmental sample enumeration (viruses, bacteria, phytoplankton and heterotrophic protists), including preservation techniques.
2. Cell Sorting for Biogeochemical analyses (C, N, & P)
3. Cell sorting for isolation and cultivation.
4. Imaging Cytometry for Aquatic applications (using FlowCAM).
5. Single cell sorting for whole genome amplification (WGA) and genome analysis.
Guest lecturers will include:
Dr. Michael Lomas, Director of the National Center for Marine Microalgae (https://ncma.bigelow.org)
Dr. Ramunas Stepanauskas, Director of the Single Cell Genomics Center (https://scgc.bigelow.org)
With additional assistance from the following Facility & Bigelow Laboratory staff members:
Dr. Steven Baer
Corporate Participants include:
Fluid Imaging Technologies
Please forward this information on to anyone interested in learning aquatic flow cytometric techniques in a laboratory-intensive week-long course on the coast of Maine this fall. For questions or additional information, or specific aquatic application requests, please contact Nicole Poulton: npoulton