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University of California Davis

Summer Short Course in Flow Cytometry

Davis, California

Sponsored by the UC Davis Biotechnology Program and the UC Davis Flow Cytometry Shared Resource

July 17-21, 2017

This comprehensive flow cytometry course is enhanced with interactive laboratory sessions using many of our UC Davis cytometers as well as new instrumentation and software to teach you to a variety of established and new approaches in cytometry. Special guest speakers will introduce new trends in flow cytometry and explain the physical and electronic concepts in light collection and signal processing. Thanks to many generous sponsors, lunches and coffee breaks are provided to promote opportunities for interaction with the instructors and cytometry specialists. Please make plans to join us this summer for another fun week of flow education.

For registration details and fees, please go to the Biotechnology Program’s summer courses page at: http://www.biotech.ucdavis.edu/SummerCourses.html

Course objectives:

· Learn the fundamental concepts of how flow cytometers work

· Discuss best practices in sample preparation for flow cytometry

· Describe standard assays and techniques

· Introduce new cutting edge technologies and applications for single cell analysis and genomics

· Demystify multicolor cell staining, compensation and analysis

Laboratory sessions include:

· UC Davis LSRII: Software setup for successful multicolor acquisition and analysis

· UC Davis Astrios Cell Sorter: Cell sorting fundamentals using the Beckman Coulter Astrios

· Cytometer setup and quality control for consistent performance with the Cytek DxP8 cytometer

· Multispectral imaging flow cytometry using the Amnis FlowSight Imaging Cytometer

· Multiparameter Analysis of Cell Surface Markers, Cytokines, Transcription Factors by Flow Cytometry: BD Biosciences on the LSRII cytometer

· FlowJo basic and advanced flow cytometry data analysis and panel design wizard

· Targeted gene expression in single cells using flow sorting and the Fluidigm BioMark

Special guest speakers:

· Holden Maecker, Ph.D., Director, Stanford University Human Immune Monitoring Core

· Jolene Bradford, Associate Director Flow Cytometry Systems, Molecular Probes, a Division of Invitrogen with ThermoFisher Scientific

· Marty Bigos, Director, Stanford Shared Flow Cytometry Laboratory

· And more!

LabFlier_2017_FlowCourse_bullets.docx