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Eisen Lab Blog
Microbial genomics job opening at Bayer CropScience
Bayer Crop Science is looking for a Microbiologist …
Eisen Lab (Science) Art Show
This past week the lab did something a bit different for lab meeting: we talked about and shared our science (and not-so-science related) art. All possible art mediums were encouraged for submission (baking, poetry, music, paintings, photographs, etc). The following slides contain the submitted art work shared at our lab meeting.
The following are descriptions of each artist’s work:
Alex Alexiev: These photographs of unidentified fungi were taken at Muir Woods National Monument in the Bay Area of California. The forest is constituted by redwoods, ferns, and small waterfalls. The high humidity creates a great habitat for various awesome fungi and molds to flourish.
Marisano James: The poem was written by Marisano when he was 19 years old and only recently mailed to him by a friend. The photographs he submitted included two dragon flies in the middle of a mating ritual, an intricately painted mailbox, a photograph of the UC Davis graduation, a dragonfly close-up and a silk moth emerging from its cocoon.
Katie Dahlhausen: These are pictures of mushroom spores taken on a scanning electron microscope that Katie built herself!
Madison Dunitz: These are images of a microbe an undergraduate student in the lab, Andrew Stump, is characterizing.
Ruth Lee: Ruth painted the two acrylic paintings and made the collage during her senior year of high school. The snow leopard was painted for a friend who had red-green color blindness. She wanted to give him something that looked the same to him as it would for everyone else. It was the first painting she ever did. The landscape painting was also a done for a friend and was the production of just her mind (no photo reference was used!). Her friend’s favorite movie was Disney’s Pocahontas, and this is her rendition of the waterfall scene. The prompt for the collage was how she thought the world should be changed and back then, Ruth thought that the best way to change the world would be to educate future generations about adopting an active approach towards the issues of today.
Hannah Holland-Moritz: Hannah enjoys amateur photography and is interested in the intersection of science photography and art. The majority of these photographs were taken on various hikes in Northern California. The microscope photo was from one of the first microbiology experiments she ever performed. It’s a biofilm stained for polysaccharides and bacterial DNA.
Environmental Sequencing workshops at #ESA2014
As part of RCN EukHiTS, I’m leading two sessions at the Ecological Society of America 2014 Annual Meeting next month (taking place in Sacramento, August 10-15, 2014) – a Sunday workshop including QIIME and metagenomics tutorials, and a special session (most likely panel discussion) on Monday. I encourage anyone who is interested to register for these events:
Workshops #9884: “Environmental sequencing approaches and computational tools for ecologists”
Sunday, August 10, 2014
8:00 AM – 5:00 PM
Description: This workshop will bring together an interdisciplinary pool of researchers to discuss current approaches, challenges, and future directions for environmental sequencing studies (-omic studies of bacteria, archaea and microbial eukaryotes). The workshop program will introduce ecologists to common data types and scientific workflows currently employed for the analysis of high-throughput sequencing data (e.g. Illumina/454). Participants will be given overview presentations and hands-on demonstrations for a number of different approaches, including rRNA marker gene analysis, shotgun metagenomics, and metatranscriptomics. This workshop will introduce participants to computational biology tools and software pipelines which can be harnessed for DNA/RNA-based “ecometagenomic” studies. In addition, we aim to solicit feedback from workshop participants, fostering discussions on how to establish better links between traditional ecological research and new, high-throughput sequencing approaches.
Special Sessions #9883: “Ecometagenomics”
Monday, August 11, 2014
10:15 AM – 11:30 AM
Description: The goal of this session is to catalyze cross-disciplinary discussions between the ecology and environmental sequencing communities. High-throughput sequencing technologies now offer tremendous opportunities to make major inroads into our understanding of global biodiversity and biogeographic patterns. However, in order to make the most of emerging high-throughput sequencing approaches, we must move towards a “systems ecology” mindset, drawing expertise from diverse disciplines. For microbial eukaryotic taxa in particular, we can now conduct en mass biodiversity assessment using traditional loci (rRNA genes) at a fraction of the time and cost required for traditional (morphological) approaches. In addition, as databases of genes with functional descriptions expand, metagenomic approaches become useful for elucidating ecosystem function. Despite this promise, current bottlenecks and roadblocks lie in the development of useful distributed tools, links between molecules and morphology/ecology, and common data standards to allow global comparisons across individual studies. This session will begin with an overview of environmental sequencing approaches, introducing participants to the methods, data types and current advantages and limitations of DNA/RNA-based studies. The session is intended to be highly interactive, including brief talks, moderated discussion points, and solicitation of questions and feedback from audience members. This session would stimulate critical discussion related to the 2014 meeting theme of “Its all ecology.” DNA/RNA-based studies represent an emerging ecological discipline, and as such, it is imperative that the growing community of microbial ecologists begins to build strong links to the traditional ecological research that forms the center point of the ESA meetings.
Another ESA session linked to RCN EukHiTS is oral session OOS10: Ecological Genomics as an Emerging Field: Opportunities for Non-model Organisms (organized by Melis Akman, UC Davis)
Paper of interest: Whole genome and exome sequencing of monozygotic twins discordant for Crohn’s disease
For those interested in microbiomes it is definitely worth looking at this paper: BMC Genomics | Abstract | Whole genome and exome sequencing of monozygotic twins discordant for Crohn’s disease
Simple summary – they have sets of identical twins where one twin has Crohn’s and the other does not. They looked for somatic mutations that could like the ones with Crohn’s and did not find any. Sure – a negative result. Could be anything. But the next obvious thing to do (which they report they are already doing) is to look at the microbioata in these people. Alas, they probably do not have data from before these people got Crohn’s, but still, this could be interesting …
Mendeley groups on environmental PCR, metagenomics, and microbial eukaryotes
As part of my NSF Research Coordination Network grant (RCN EukHiTS), I am currently managing a number of Mendeley groups that amalgamate relevant journal articles on different topics related to environmental PCR, metagenomics, and microbial eukaryotes. These groups are open (anyone can join with a Mendeley account), and I’m trying to keep them regularly updated with new articles (Mendeley members can also add articles, which I strongly encourage!):
- Eukaryotic HTP Studies – Publications relevant to high-throughput environmental sequencing approaches focused on microbial eukaryotes. Articles will include any type of -Omic methods (marker gene amplicons, metagenomics, metatranscriptomics, etc.), eukaryote-focused tools/pipelines, and review/opinion pieces.
- rRNA in Eukaryotes – Literature related to the ribosomal repeat array in eukaryotic genomes – variation in rRNA gene copy number, intragenomic polymorphisms, concerted evolution, transposable elements and their evolutionary and ecological implications.
- Environmental PCRs – primer sets and bias – Literature related to primer set usage and bias across all taxonomic groups (bacteria, archaea, fungi and microbial eukaryotes) – includes primer sets and methods focused on 16S, 18S, ITS, other rRNA, COI, and other marker genes used for environmental sequencing.
- eDNA in aquatic ecosystems – This group focuses on environmental DNA (eDNA) applications in aquatic ecosystems, include use of eDNA in bioassessment and environmental monitoring. Literature collection covers methods, analytical tools, and empirical studies (both basic and applied science).
Pacific Northwest National Lab (PNNL) is looking for some microbial computational / genomics folks
Check out these job ads. PNNL is looking for a few good microbial people.
Postdoc Flyer.pdf
PNNL Assoc Director Microbiology.pdf
Fun times with whooping cough in #DavisCA
Just got this email. I have removed the specific sender / facility since that does not seem needed for my purposes here. I have replaced the name of the site with “Our Facility”. Anyway – thought some people would be interested in the things that can happen when too many people in your community do not vaccinate their kids.
From: Office AdministrationSubject: Possible Exposure to Whooping Cough – PertussisDate: June 25, 2014 at 12:55:59 PM PDTTo: Office AdministrationDear Parent or Guardian,Our Facility was informed this morning that a student who has been attending a class since 6/16/14 has been diagnosed with pertussis (whooping cough). The child is no longer attending the class and is under a medical care.If you feel you or your child may have been exposed, watch carefully for symptoms in the next 1-2 weeks, and consult a physician if any occur. Here is some pertinent information regarding pertussis (whooping cough):Pertussis can spread through the air when people cough. It often starts like a common cold which gets worse and worse over 1-2 weeks. People with pertussis have coughing spells that may last several seconds. As they catch their breath at the end of each coughing spell, they may gasp loudly (“whoop”) and vomit or choke.The vaccine usually protects against pertussis, but sometimes even immunized children can get pertussis. Pertussis is treated with antibiotics. Antibiotics can also prevent the spread of pertussis to others.Activities that may help stop the spread of pertussis include:
- The exclusion of all pertussis cases from classes until they have taken the first five days of the antibiotic.
- The administration of antibiotics for babies, pregnant women and other high risk contacts of pertussis cases as well as consideration of antibiotic administration for other household or close contacts to prevent the further spread of pertussis.
- The administration of another dose of DTaP vaccine to babies and Tdap for older children who are not up to date on their vaccine series.
Please know that Our Facility is taking every precaution in this matter and let us know if you have any questions or concerns.Sincerely,Office Staff
“An Evening with the World’s Leading Marine Microbiologists” in San Jose
Just got this and am sharing:
Dear Dr. Eisen,
The Tech museum in San Jose wanted to invite you to a very special event of ours that might be of your interest. On July 30th, 16 exceptional marine microbiologists from all over the country will gather in The Tech for an evening of conversation with our local community.
The Tech would love for you to join us! I attached a more detailed description of the event below. Please don’t hesitate to contact me if you have any questions or concerns. And we would also highly appreciate if you could forward this email to any of your colleagues and students who might be interested in coming.
Thank you so much and look froward to hearing back from you!
Best Regards,
Haven Yang
The Tech Museum of Innovation
201 S. Market St., San Jose, CA 95113 | thetech.org
Exclusive Engagement!
An Evening with the World’s Leading Marine Microbiologists
A Creative Collisions Initiative
Wednesday, July 30, 2014
5:45 p.m.
The Tech Museum of Innovation’s Lower Level
General admission $25 in advance/$35 at the door
Members $15/$25
Students and Seniors $10/$15
Food & Beverage available for sale at event
Buy Tickets
“The oxygen in every second breath we take is produced by microbes in our oceans—they are thought to produce about 50 percent of the oxygen in the earth’s atmosphere. Yet, we know surprisingly little about these tiny organisms.”
—The Gordon and Betty Moore Foundation’s Marine Microbiology Initiative
The Tech welcomes 16 world-renowned scientists for an evening of discussion and discovery.
Please join us to learn more about our diverse ocean ecosystems through robust conversation with some of the most innovative minds of our time, and to connect with bio-focused community groups and individuals. Listen to Professor Kay Bidle of Rutgers University explore “The Invisible World of Marine Microbes: How Earth’s Smallest Living Things Have the Biggest Impact on How Our Ocean Works.”
Mingle with the 16 Investigators of the Gordon and Betty Moore Foundation’s Marine Microbiology Initiative:
- Andrew Allen, J. Craig Venter Institute
- Ginger Armbrust, University of Washington
- Kay Bidle, Rutgers University
- Edward DeLong, Massachusetts Institute of Technology
- Curtis Deutsch, University of Washington
- Nicole Dubilier, Max Planck Institute for Marine Microbiology
- Michael Follows, Massachusetts Institute of Technology
- Jed Fuhrman, University of Southern California
- David Karl, University of Hawaii
- Victoria Orphan, California Institute of Technology
- Ann Pearson, Harvard University
- Forest Rohwer, San Diego State University
- Mak Saito, Woods Hole Oceanographic Institution
- Roman Stocker, Massachusetts Institute of Technology
- Matthew Sullivan, University of Arizona
- Alexandra Worden, Monterey Bay Aquarium Research Institute
| Schedule | |
| 5:45—6:45 p.m. | Mingling over food, drinks and exhibits |
| 6:45—7:30 p.m. | Kay Bidle: “The Invisible World of Marine Microbes: How Earth’s Smallest Living Things Have the Biggest Impact on How Our Ocean Works,” followed by 15-minute Q&A |
| 7:30—10 p.m. | More mingling over food and drinks |
